No apparent difference in lactotrope distribution was observed between dKO mice and WT controls. Immunohistochemistry for PRL was performed on pituitary gland tissue from female and male mice to determine the distribution of lactotropes. Lactotrope cells appear normally distributed in dKO mice. Representative images of three animals per genotype and sex are shown. No obvious difference was observed between dKO mice and WT controls. Immunohistochemistry for LHB, TSHB, and ACTH was performed on pituitary gland tissue from female and male mice to determine the distribution of gonadotropes, thyrotropes, and corticotropes, respectively. Gonadotrope, thyrotrope and corticotrope cells appear normally distributed in dKO mice. The data represent 7-8 animals for each genotype and sex and were analyzed using Student’s t test. Hypothalamus was collected to evaluate expression of Sst. RNA was isolated and cDNA generated in order to evaluate mRNA abundance for Prop1 in females and males. Whole pituitary glands were collected from WT and dKO mice at 6 weeks of age. Prop1 and Sst expression is unchanged in dKO mice.
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